LOCUS       40924_49072        3400 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD012, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    370..375
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(373..1425)
                     /note="CVD-ANTR; device for the GC-adaptor assembly:
                     antibiotic resistance markers"
     misc_feature    complement(373..393)
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    394..399
                     /label=AgeI
                     /note="AgeI"
     regulatory      406..562
                     /note="PnptII; upstream region of nptII ORF including
                     promoter and RBS in INSD accession V00618; derived from 
                     pAM505"
                     /regulatory_class="promoter"
     CDS             563..1354
                     /codon_start=1
                     /product="nptII_A7120"
                     /label=nptII_A7120
                     /note="confers kanamycin resistance; aminoglycoside 
                     phosphotransferase"
                     /protein_id="AII99780.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      1363..1389
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    1399..1404
                     /label=NheI
                     /note="NheI"
     misc_feature    complement(1405..1425)
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1423..1428
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1653..2241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2415..3272)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3273..3377)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatg atatccggcg gcggcggcgg gataccggtt tcgaatttat ggacagcaag
      421 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
      481 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga
      541 gacaggatga ggatcgtttc gcatgattga acaagatggt ttacatgctg gtagtccagc
      601 tgcttgggtt gaacgtttat ttggttatga ttgggctcaa caaaccattg gttgtagtga
      661 tgctgctgtt tttcgtttaa gtgctcaagg tcgtcccgtg ttatttgtga aaactgattt
      721 aagcggtgct ttaaatgaat tacaagatga agctgctcgc ttgagttggt tagctactac
      781 tggtgttcca tgtgctgctg tattagatgt tgttactgaa gctggtcgtg attggttatt
      841 gttaggtgaa gttccaggtc aagatttatt gagtagtcat ttagctcccg ctgaaaaagt
      901 gagtattatg gctgatgcta tgcgtcgctt acatacttta gatccagcta cttgtccctt
      961 tgatcatcaa gctaaacatc gtattgaacg tgctcgtact cgtatggaag ctggtttagt
     1021 tgatcaagat gatttagatg aagaacatca aggtttagcc ccagctgaat tatttgctcg
     1081 tttaaaagct cgtatgcccg atggtgaaga tttagttgtt actcatggtg atgcctgttt
     1141 gcccaatatt atggttgaaa atggtcgctt tagtggtttt attgattgtg gtcgtttagg
     1201 tgttgctgat cgctatcaag atattgcttt agctactcgc gatattgccg aagaattagg
     1261 tgaatgggct gatcgctttt tagtgttata tggtattgct gctccagata gtcaacgcat
     1321 tgctttttat cgcttattag atgaattttt ttaaggccgg ccagcccgcc taatgagcgg
     1381 gctttttttt ggcgcgccgc tagcatccgc gcgcgcgcgc gcgatatcct gggcctcatg
     1441 ggccttccgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca
     1501 tggtcatagc tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct
     1561 gcgctcggtc gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc
     1621 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1681 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1741 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1801 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1861 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1921 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1981 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2041 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2101 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2161 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2221 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2281 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2341 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2401 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2461 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2521 ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta
     2581 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2641 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2701 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2761 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2821 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2881 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2941 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3001 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3061 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3121 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3181 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3241 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3301 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3361 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
